How to replace/fill "Ns" in fasta with reference file having same coordinates

Hope you are doing great. As asked in title, please guide if there is any way to fill or replace N or N's in fasta file with the help of reference file.

For example


Fasta with Ns


Reference Fasta




I will really appreciate any help in this regard

1 answer

  • answered 2021-11-27 19:26 Ed Morton

    If this (tongue in cheek) isn't all you need:

    $ head -1 FastaWithNs; tail -1 ReferenceFasta

    then edit your question to better describe your requirements and provide more truly representative sample input/output.

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